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Bacillus cavernae sp. nov. isolated from cave soil (Feng liling)

Liling Feng, Dongmei Liu, Xuelian Sun, Gejiao Wang3 and Mingshun Li3

A Gram-stain-positive, spore-forming, motile, strictly aerobic, rod-shaped bacterium, designated
strain L5T, was isolated from soil of Tenglong cave, China. 16S rRNA gene sequence analysis
showed that strain L5T was related most closely to Bacillus asahii MA001T (96.5 %)
(the highest 16S rRNA gene sequence similarity), Bacillus kribbensis BT080T (96.4 %) and
Bacillus deserti ZLD-8T (96.2 %). The DNA G+C content of strain L5T was 45.6 mol%.
The major menaquinone was MK-7. The major fatty acids were iso-C14 : 0, anteiso-C15 : 0 and
iso-C16 : 0, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and
phosphatidylethanolamine. The diagnostic diamino acid in the cell-wall peptidoglycan was
meso-diaminopimelic acid. In addition, strain L5T had different characteristics compared with
the other Bacillus strains such as pink colony colour, low growth temperature and low nutrient
requirement. The results indicate that strain L5T represents a novel species of the genus
Bacillus, for which the name Bacillus cavernae sp. nov. is proposed. The type strain is L5T
(5KCTC 33637T5CCTCC AB 2015055T).



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